pooled serum वाक्य
उदाहरण वाक्य
मोबाइल
- Generally , the overall researches can be summarized to the following phases : 1 . ak auto ab was purified from human pooled sera by affinity chromatography
- After absorbed with protein g - sepharose beads to remove the igg antibody , the pooled sera were then used for immunoscreening of the cdna library of schistosoma japonicum adult worms
- Then , the gene was cloned into vector pgem - t and subcloncd into expression vector pgex - 6p - l and induced by iptg . western blot analysis showed that the fusion protein expressed could be recognized by the specific ige antibody in the pooled sera
- The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed . the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold . in order to further analyse the epitope features of the protein relevant to the specific ige antibody , the phage display library of random peptides was screened by pooled sera with the specific ige antibody
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